Can IDEXX Angio Detect™ accurately detect canine Angiostrongylosis?



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PICO question

In dogs, is IDEXX Angio Detect™ as accurate as Baermann coprology when diagnosing Angiostrongylus vasorum infection?


Clinical bottom line

Category of research question


The number and type of study designs reviewed

Eight papers were critically reviewed: three diagnostic accuracy studies, two cross-sectional studies (one of which also included a retrospective case series), one cohort study, one case-control study, and one case series

Strength of evidence


Outcomes reported

Angio Detect™ (IDEXX) was shown to have low-moderate sensitivity and high specificity in comparison to Baermann coprology. Occasionally, false-negative results occurred with Angio Detect™ when compared to Baermann coprology. This was thought to be due to antigen-antibody complex formation. Positive Angio Detect™ assays were obtained in both symptomatic and asymptomatic canine patients. In an experimental setting, Angio Detect™ was shown to obtain a positive result five weeks post-inoculation


There is weak evidence supporting Angio Detect™ as a highly specific and moderately sensitive diagnostic test when compared to Baermann coprology


How to apply this evidence in practice

The application of evidence into practice should take into account multiple factors, not limited to: individual clinical expertise, patient’s circumstances and owners’ values, country, location or clinic where you work, the individual case in front of you, the availability of therapies and resources.

Knowledge Summaries are a resource to help reinforce or inform decision making. They do not override the responsibility or judgement of the practitioner to do what is best for the animal in their care.


Open Access Peer Reviewed


Barçante, J.M.P., Barçante, T.A., Ribeiro, V.M., Oliveira-Junior, S.D., Dias, S.R.C., Negrão-Corrêa, D. & Lima, W.S. (2008). Cytological and parasitological analysis of bronchoalveolar lavage fluid for the diagnosis of Angiostrongylus vasorum infection in dogs. Veterinary Parasitology. 158(1–2), 93–102. DOI:

Bourne, D., Elsheikha, H., Farquhar, R., Helm, J., Morgan, E., Peters, I., Sturgess, K., Torrance, A. & Wright, I. (2020). Lungworm: A roundtable discussion. Companion Animal. 25(2), 65–75. DOI:

Broussard, J.D. (2003). Optimal fecal assessment. Clinical Techniques in Small Animal Practice. 18(4), 218–230. DOI:

Bujang, M.A. & Adnan, T.H. (2016). Requirements for Minimum Sample Size for Sensitivity and Specificity Analysis. Journal of Clinical and Diagnostic Research. 10(10), YE01–YE06. DOI:

Canonne, A.M., Billen, F., Losson, B., Peters, I. Schnyder, M. & Clercx, C. (2018). Angiostrongylosis in dogs with negative fecal and in-clinic rapid serological tests: 7 Cases (2013–2017). Journal of Veterinary Internal Medicine. 32(3), 951–955. DOI:

Cohen, J.F., Korevaar, D.A., Altman, D.G., Bruns, D.E., Gatsonis, C.A., Hooft, L., Irwig, L., Levine, D., Reitsma, J.B., De Vet, H.C. & Bossuyt, P.M. (2016). STARD 2015 guidelines for reporting diagnostic accuracy studies: explanation and elaboration. BMJ Open. 6(11), e012799. DOI:

Conboy, G. (2009). Helminth Parasites of the Canine and Feline Respiratory Tract. Veterinary Clinics of North America: Small Animal Practice. 39(6), 1109–1126. DOI:

Di Cesare, A., Miotti, C., Venco, L., Pampurini, F., Centaro, E. & Traversa, D. (2014). Subclinical Angiostrongylus vasorum Infection In A Terrier Dog Kennel. Polish Journal of Natural Sciences. 29(2), 189–195.

Di Cesare, A., Traversa, D., Manzocchi, S., Meloni, S., Grillotti, E., Auriemma, E., Pampurini, F., Garofani, C., Ibba, F. & Venco, L. (2015) Elusive Angiostrongylus vasorum Parasites & Vectors. 8 (1), 438–447. DOI:

Elsheikha, H., Holmes, S.A., Wright, I., Morgan, E.R. & Lacher, D.W. (2014). Recent advances in the epidemiology, clinical and diagnostic features, and control of canine cardio-pulmonary angiostrongylosis. Veterinary Research. 45(92), 1–12. DOI:

Humm, K. & Adamantos, S. (2010). Is evaluation of faecal smear a useful technique in the diagnosis of canine pulmonary angiostrongylosis. The Journal of Small Animal Practice. 51(4), 200–203. DOI:

Koch, J. & Willesen, J.L. (2009). Canine pulmonary angiostrongylosis: An update. The Veterinary Journal. 179(3), 348–359. DOI:

Lempereur, L., Martinelle, L., Marechal, F., Bayrou, C., Dalemans, A.C., Schnyder, M. & Losson, B. (2016). Prevalence of Angiostrongylus vasorum in southern Belgium, a coprological and serological survey. Parasites & Vectors, 9(1), 1–7. DOI:

Lempereur, L., Nijsse, R., Losson, B., Marechal, F., De Volder, A., Schoormans, A., Martinelle, L., Danlois, F. & Claerebout, E. (2020). Coprological survey of endoparasite infections in owned dogs and owners’ perceptions of endoparasite control in Belgium and the Netherlands. Veterinary Parasitology: Regional Studies and Reports. 22, 100450. DOI:

Liu, J., Schnyder, M., Willesen, J.L., Potter, A. & Chandrashekar, R. (2017). Performance of the Angio Detect™ in-clinic test kit for detection of Angiostrongylus vasorum infection in dog samples from Europe. Veterinary Parasitology: Regional Studies and Reports. 7, 45–47. DOI:

McGarry, J.W. & Morgan, E.R. (2009). Identification of first-stage larvae of metastrongyles from dogs. The Veterinary Record. 165(9), 258–261. DOI:

Naeger, D.M., Kohi, M.P., Webb, E.M., Phelps, A., Ordovas, K.G. & Newman, T.B. (2013). Correctly Using Sensitivity, Specificity, and Predictive Values in Clinical Practice: How to Avoid Three Common Pitfalls. American Journal of Roentgenology. 200(6), W566–W570. DOI:

Oliveira-Júnior, S.D., Barçante, J.M.P., Barçante, T.A., Dias, S.R.C. & Lima, W.S. (2006). Larval output of infected and re-infected dogs with Angiostrongylus vasorum (Baillet, 1866) Kamensky, 1905. Veterinary Parasitology. 141(1–2), 101–106. DOI:

Olivieri, E., Zanzani, S.A., Gazzonis, A.L., Giudice, C., Brambilla, P., Alberti, I., Romussi, S., Lombardo, R., Mortellaro, C.M., Banco, B., Vanzulli, F.M., Veronesi, F. & Manfredi, M.T. (2017). Angiostrongylus vasorum infection in dogs from a cardiopulmonary dirofilariosis endemic area of Northwestern Italy: a case study and a retrospective data analysis. BMC Veterinary Research. 13(1), 1–7. DOI:

Schnyder, M., Stebler, K., Naucke, T.J., Lorentz, S. & Deplazes, P. (2014). Evaluation of a rapid device for serological in-clinic diagnosis of canine angiostrongylosis. Parasites & Vectors. 7(1), 1–7. DOI:

M., Tanner, I., Webster, P., Barutzki, D. & Deplazes, P. (2011). An ELISA for sensitive and specific detection of circulating antigen of Angiostrongylus vasorum in serum samples of naturally and experimentally infected dogs. Veterinary Parasitology. 179(1–3), 152–158. DOI:

Sergeant, E.S.G. (2018) Epitools Epidemiological Calculators. Ausvet. Available at: [Accessed 06/05/2021]

Swift, A., Heale, R. & Twycross, A. (2020). What are sensitivity and specificity? Evidence-Based Nursing. 23(1), 2–4. DOI:

Traversa, D., Di Cesare, A. & Conboy, G. (2010). Canine and feline cardiopulmonary parasitic nematodes in Europe: emerging and underestimated. Parasites & Vectors. 3(62), 1–22. DOI:

Venco, L., Colaneri, G., Formaggini, L., De Franco, M. & Rishniw, M. (2021). Utility of thoracic ultrasonography in a rapid diagnosis of angiostrongylosis in young dogs presenting with respiratory distress. The Veterinary Journal. 271(105649), 1–6. DOI:

Verzberger-Epshtein, I., Markham, R.J.F., Sheppard, J.A., Stryhn, H., Whitney, H. & Conboy, G.A. (2008). Serologic detection of Angiostrongylus vasorum infection in dogs. Veterinary Parasitology. 1(25), 53–60. DOI:

Vol. 6 No. 4 (2021): The fourth issue of 2021

Section: Knowledge Summaries

Categories :  Small Animal  /  Dogs  /  Cats  /  Rabbits  /  Production Animal  /  Cattle  /  Sheep  /  Pig  /  Equine  /