Does use of dog-appeasing pheromone reduce the frequency and/or severity of non-specific stress behaviours associated with anxiety in domestic dogs, older than 6 months, when compared with no treatment?
Clinical bottom line
Category of research question
The number and type of study designs reviewed
Eight controlled trials were appraised. Four were randomised and four were either non-randomised or did not clearly describe the method of allocating subjects into treatment groups.
Strength of evidence
There was no evidence that any dog appeasing pheromone (DAP) formulation (spray, diffuser, or collar) was superior. There was moderate evidence that DAP could reduce some behavioural manifestations of fear and/or anxiety stemming from thunderstorm noise and weak evidence that it could ameliorate some non-specific stress behaviours in hospitalised patients. In shelter dogs, there was mild evidence that DAP could reduce barking intensity and increase some behaviours associated with relaxation. When behavioural changes occurred, most were observed during exposure to DAP and there were minimal residual effects post-treatment.
The evidence for using DAP to manage stress behaviours associated with anxiety in dogs over six months of age remains weak. Until there is a stronger evidentiary basis, clinicians should be aware that a true clinical benefit is undetermined. Nevertheless, DAP is unlikely to cause harm and may still provide some therapeutic benefit. Therefore, DAP may still be employed in a multimodal management plan for some behaviour cases and may exert a placebo effect. However, if an owner’s financial resources are restrictive, clinicians should not prioritise pheromone therapy at the omission of other therapies that have established clinical effects
The application of evidence into practice should take into account multiple factors, not limited to: individual clinical expertise, patient’s circumstances and owners’ values, country, location or clinic where you work, the individual case in front of you, the availability of therapies and resources.
Knowledge Summaries are a resource to help reinforce or inform decision making. They do not override the responsibility or judgement of the practitioner to do what is best for the animal in their care.
Amaya, V., Paterson, M. B. A. & Phillips, C. J. C. (2020). Effects of Olfactory and Auditory Enrichment on the Behaviour of Shelter Dogs. Animals. 10(4), 581. DOI: https://doi.org/10.3390/ani10040581
Beata, C., Beaumont-Graff, E., Diaz, C., Marion, M., Massal, N., Marlois, N., Muller, G. & Lefranc, C. (2007). Effects of alpha-casozepine (Zylkene) versus selegiline hydrochloride (Selgian, Anipryl) on anxiety disorders in dogs. Journal of Veterinary Behavior. 2(5), 175-183. DOI: https://doi.org/10.1016/j.jveb.2007.08.001
Beerda, B., Schilder, M. B. H., van Hooff, J. A. R. A. M., de Vries, H. W. & Mol, J. A. (1998). Behavioural, saliva cortisol and heart rate responses to different types of stimuli in dogs. Applied Animal Behaviour Science. 58(3-4), 365-381. DOI: https://doi.org/10.1016/s0168-1591(97)00145-7
Berger, V. W. & Grant, W. (2014). A review of randomization methods in clinical trials. In: W.R. Young & D.-G.D. Chen, eds., Clinical Trial Biostatistics and Biopharmaceutical Applications, 1st ed. London: CRC Press LLC, 41-54.
Bradshaw, J. W. S. & Casey, R. A. (2007). Anthropomorphism and anthropocentrism as influences in the quality of life of companion animals. Animal Welfare. 16(S1), 149-154. [online] Available from: https://www.ingentaconnect.com/content/ufaw/aw/2007/00000016/a00102s1/art00022 [Accessed 07 Aug 2020].
Broach, D. & Dunham, A. E. (2016). Evaluation of a pheromone collar on canine behaviors during transition from foster homes to a training kennel in juvenile Military Working Dogs. Journal of Veterinary Behavior. 14(July–August), 41-51. DOI: https://doi.org/10.1016/j.jveb.2016.05.001
Burghardt, G. M., Bartmess‐LeVasseur, J. N., Browning, S. A., Morrison, K. E., Stec, C. L., Zachau, C. E. & Freeberg, T. M. (2012). Perspectives–minimizing observer bias in behavioral studies: a review and recommendations. Ethology. 118(6), 511-517. DOI: https://doi.org/10.1111/j.1439-0310.2012.02040.x
Ceva Animal Health Pty Ltd. (n.d.). FAQ. Available at: https://www.adaptil.com/au/Products/FAQ [Accessed 07 Sep 2020].
Dietz, L., Arnold, A.-M. K., Goerlich-Jansson, V. C. & Vinke, C. M. (2018). The importance of early life experiences for the development of behavioural disorders in domestic dogs. Behaviour. 155(2-3), 83-114. DOI: https://doi.org/10.1163/1568539X-00003486
Fives, A., Rusell, D. W., Kearns, N., Lyons, R., Eaton, P., Canavan, J., Devaney, C. & O'Brien, A. (2013). The role of random allocation in randomized controlled trials: distinguishing selection bias from baseline imbalance. Journal of MultiDisciplinary Evaluation. 9(20), 33-42. [online] Available from: https://journals.sfu.ca/jmde/index.php/jmde_1/article/view/371 [Accessed 17 Aug 2020].
Frank, D., Beauchamp, G. & Palestrini, C. (2010). Systematic review of the use of pheromones for treatment of undesirable behavior in cats and dogs. J Am Vet Med Assoc. 236(12), 1308-1316. DOI: https://doi.org/10.2460/javma.236.12.1308
Girard, J. M. & Cohn, J. F. (2016). A Primer on Observational Measurement. Assessment. 23(4), 404-413. DOI: https://dx.doi.org/10.1177%2F1073191116635807
Grigg, E. K. & Piehler, M. (2015). Influence of dog appeasing pheromone (DAP) on dogs housed in a long-term kennelling facility. Vet Rec Open. 2(1), e000098. DOI: http://dx.doi.org/10.1136/vetreco-2014-000098
Hammerle, M., Horst, C., Levine, E., Overall, K., Radosta, L., Rafter-Ritchie, M. & Yin, S. (2015). 2015 AAHA Canine and Feline Behavior Management Guidelines. J Am Anim Hosp Assoc. 51(4), 205-221. DOI: https://doi.org/10.5326/JAAHA-MS-6527
Harvey, N. D. (2021). How Old Is My Dog? Identification of Rational Age Groupings in Pet Dogs Based Upon Normative Age-Linked Processes. Front Vet Sci. 8(321), 643085. DOI: https://doi.org/10.3389/fvets.2021.643085
Hermiston, C., Montrose, V. T. & Taylor, S. (2018). The effects of dog-appeasing pheromone spray upon canine vocalizations and stress-related behaviors in a rescue shelter. Journal of Veterinary Behavior. 26(July-August), 11-16. DOI: https://doi.org/10.1016/j.jveb.2018.03.013
Kaufman, A. B. & Rosenthal, R. (2009). Can you believe my eyes? The importance of interobserver reliability statistics in observations of animal behaviour. Animal Behaviour. 78(6), 1487-1491. DOI: https://psycnet.apa.org/doi/10.1016/j.anbehav.2009.09.014
Kim, Y. M., Lee, J. K., Abd el-aty, A. M., Hwang, S. H., Lee, J. H. & Lee, S. M. (2010). Efficacy of dog-appeasing pheromone (DAP) for ameliorating separation-related behavioral signs in hospitalized dogs. Can Vet J. 51(4), 380-384. [online] Available from: https://www.ncbi.nlm.nih.gov/pubmed/20592826 [Accessed 24 June 2021].
Landsberg, G. M., Beck, A., Lopez, A., Deniaud, M., Araujo, J. A. & Milgram, N. W. (2015). Dog-appeasing pheromone collars reduce sound-induced fear and anxiety in beagle dogs: a placebo-controlled study. Vet Rec. 177(10), 260. DOI: http://dx.doi.org/10.1136/vr.103172
Lund, J. D. & Jørgensen, M. C. (1999). Behaviour patterns and time course of activity in dogs with separation problems. Applied Animal Behaviour Science. 63(3), 219-236. DOI: https://doi.org/10.1016/S0168-1591(99)00011-8
Miller, D. D., Staats, S. R., Partlo, C. & Rada, K. (1996). Factors associated with the decision to surrender a pet to an animal shelter. J Am Vet Med Assoc. 209(4), 738-742. [online] Available from: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030586603&partnerID=40&md5=d7816937c703fcb27da23906255e3d12 [Accessed 08 Aug 2020].
Mills, D. S. (2017). Perspectives on assessing the emotional behavior of animals with behavior problems. Current Opinion in Behavioral Sciences. 16(August), 66-72. DOI: https://doi.org/10.1016/j.cobeha.2017.04.002
O'Connor, A. M., Sargeant, J. M. & Wang, C. (2014). Conducting systematic reviews of intervention questions III: Synthesizing data from intervention studies using meta-analysis. Zoonoses Public Health. 61(S1), 52-63. DOI: https://doi.org/10.1111/zph.12123
Overall, K. (2013). Manual of Clinical Behavioral Medicine for Dogs and Cats-E-Book. Elsevier Health Sciences. [online] Available from: https://ebookcentral-proquest-com.ezproxy.library.sydney.edu.au/lib/usyd/detail.action?docID=1431145 [Accessed 30 Jul 2020].
Pageat, P. & Gaultier, E. (2003). Current research in canine and feline pheromones. Vet Clin North Am Small Anim Pract. 33(2), 187-211. DOI: https://doi.org/10.1016/S0195-5616(02)00128-6
Paul, E. S., Harding, E. J. & Mendl, M. (2005). Measuring emotional processes in animals: the utility of a cognitive approach. Neurosci Biobehav Rev. 29(3), 469-91. DOI: https://doi.org/10.1016/j.neubiorev.2005.01.002
Pierotti, P. (2016). Clinical applications of pheromones in dogs. Dog Behavior. 2(2), 20-26. DOI: https://doi.org/10.4454/db.v2i2.32
Prior, M. R. & Mills, D. S. (2020). Cats vs. Dogs: The Efficacy of Feliway Friends(TM) and Adaptil(TM) Products in Multispecies Homes. Front Vet Sci. 7(399). DOI: https://dx.doi.org/10.3389/fvets.2020.00399
Protopopova, A. (2016). Effects of sheltering on physiology, immune function, behavior, and the welfare of dogs. Physiol Behav. 159(15 May 2016), 95-103. DOI: https://doi.org/10.1016/j.physbeh.2016.03.020
Salonen, M., Sulkama, S., Mikkola, S., Puurunen, J., Hakanen, E., Tiira, K., Araujo, C. & Lohi, H. (2020). Prevalence, comorbidity, and breed differences in canine anxiety in 13,700 Finnish pet dogs. Sci Rep. 10(1), 2962. DOI: https://doi.org/10.1038/s41598-020-59837-z
Scott, J. & Fuller, J. (1965). Genetics and social behavior of the dog. University of Chicago Press. Chicago.
Siracusa, C., Manteca, X., Cuenca, R., del Mar Alcala, M., Alba, A., Lavin, S. & Pastor, J. (2010). Effect of a synthetic appeasing pheromone on behavioral, neuroendocrine, immune, and acute-phase perioperative stress responses in dogs. J Am Vet Med Assoc. 237(6), 673-81. DOI: https://doi.org/10.2460/javma.237.6.673
Sueda, K. L. & Malamed, R. (2014). Canine aggression toward people: a guide for practitioners. Vet Clin North Am Small Anim Pract. 44(3), 599-628. DOI: https://doi.org/10.1016/j.cvsm.2014.01.008
Taborsky, M. (2010). Sample size in the study of behaviour. Ethology. 116(3), 185-202. DOI: https://doi.org/10.1111/j.1439-0310.2010.01751.x
Taylor, S., Webb, L., Montrose, V. T. & Williams, J. (2020). The behavioral and physiological effects of dog appeasing pheromone on canine behavior during separation from the owner. Journal of Veterinary Behavior. 40(November-December), 36-42. DOI: https://doi.org/10.1016/j.jveb.2020.08.001
Tiira, K., Sulkama, S. & Lohi, H. (2016). Prevalence, comorbidity, and behavioral variation in canine anxiety. Journal of Veterinary Behavior. 16(November-December), 36-44. DOI: https://doi.org/10.1016/j.jveb.2016.06.008
Wemelsfelder, F. (2001). The Inside and Outside Aspects of Consciousness: Complementary Approaches to the Study of Animal Emotion. Animal Welfare. 10(1), 129-139. DOI: https://www.ingentaconnect.com/content/ufaw/aw/2001/00000010/a00101s1/art00012 [Accessed 07 Aug 2020]
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